It is true, it dissolves during prophase because it is not needed in the following phase of metaphase
Answer:
if the promoterless lacZ is inserted close to any gene(s) or operon, then the laz gene will also be transcribed when the operon gets expressed. The polycystronic RNA will eventually produce a beta-galactosidase protein(product of lacZ gene) which will cleave X-Gal( structural analog to lactose), which will produce blue chromogenic substance.
prepare LB agar. Allow it to cool, add X-gal before it solidifies, pour to plates.
create grids and patch plate each strain on it. Just pick the strain that looks blue, amplify the sequence upstream of the lacZ and sequence it.
Explanation:
Just pick the strain that looks blue, amplify the sequence upstream of the lacZ, and sequence it.
B. Air temperature
Because as air temperature increase the global climate change will increase with it causing glaciers to melt, wild fires to increase, dry air, more plants dying, and droughts...
