I'm sorry, but is this a question? I'm a bit confused.
The 3rd,4th, and 5th are correct.
An intramuscular injection is a technique delivering a medication that is deep into the muscles. If intramuscular is administered too high then the efficacy of the vaccine might be reduced as it is not injected in the right site. It might also cause to injure blood vessels so it should be injected in the correct site and depth.
I believe this difference in concentration of dissolved Particles from one location to another is called a concentration gradient.
A) the DNA condenses and the nuclear membrane breaks