I'm sorry but I cannot answer this question due to the fact that it has nothing to do with J.R.R Tolien XDXDXD
Explanation:
Conservation of Energy in Living Systems
Energy changes form as it flows through an ecosystem, but the total amount of energy in the system remains constant. Food chains show how energy flows in an ecosystem. ... The chemical energy stored in producers is passed to consumers, organisms at higher levels on the food chain.
Answer:
Based on the given information, the disruption of hydrogen bonds and other weak forces that helps in integrating the structure of the protein is mediated by guanidine hydrochloride, that is, it denatures the protein. Only a solitary peak of Mr 60000 is witnessed post denaturation. This clearly shows that only one peptide of Mr 60000 is left in the solution post denaturation.
Of the entire protein of molecular weight 240000 D, post denaturation only the protein of 60000 D got left, which shows that the protein comprises four subunits of 60000 D, and thus, is a tetramer.
With the addition of 10 mM beta-mercaptoethanol, the protein got further dissociated into two subunits of molecular weight 34000 D and 26000 D showing that each subunit of protein was linked by disulfide bonds, which got broken with the addition of beta-mercaptoethanol.
