Answer:
DNA restriction enzymes cut the DNA molecule, while DNA ligases join the resulting DNA fragments
Explanation:
Transformation is a naturally occurring process by which bacteria incorporate exogenous genetic material from their surrounding environment. This process (transformation) is used for DNA cloning via plasmid vectors. In DNA cloning, transformation occurs after restriction enzymes cut the DNA at specific sequences named palindromic sequences (i.e, sequences that can be read the same in opposite direction). Restriction enzymes can generate sticky-ends, where enzymes make staggered cuts in the two strands (e.g., <em>BamH</em>), or blunt ends, where the resulting strands are of the same length (e.g., <em>HaeIII</em>). In general, sticky-end enzymes are more useful because they generate a 3' overhang in one molecule and a complementary 5' overhang in the other, increasing the yield and specificity of ligation. During ligation, a DNA ligase is used to join both DNA strands by forming phosphodiester bonds in the plasmid. Following transformation, bacteria can be selected on antibiotic plates.
TRUE: plants have chloroplasts, which serves as a glucose source within the plant. FALSE: both plant and animal cells have a cell membrane, but animal cells do not have cell walls. ... FALSE: Eukaryotes have a defined, membrane-bound nucleus while prokaryotes do not.
If they are negative and positive then they are attraction if the are the same then they are repulsion.
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Answer:
The most likely cause of Nota’s pain is shoulder dislocation/ subluxation.
Explanation:
When Laika picked Nota up by the right arm and lifts him, the shoulder was in abduction and external rotation, which favours shoulder dislocation. This was supported by the loud popping sound, guarding of right arm and pain, which is severe in nature.
