Answer:
Explanation:
Bacterial count in stock- 1.85x10^6 cfu/ml
Dilution methods
Take 100 uL or (0.1ml) from stock and add to 900ul (0.9ml) saline and mixed it- this makes 10^1dilution.
Now take 100ul from 10^1 dilution and add to next 900ul saline this is 10^2 dilution, similarly do upto 10^5 dilution.
Then take 100ul from 10^ 4 and 10^5 dilution seperately and plate on LB agar plate seperetely and count the colonies.
Cfu/ml formula= (No.of colonies x dilution factor)/0.1 ml
So suppose, 18 colonies formed on 10^4 dilution then total no. Of cells in stock will be 18x10^4/ 0.1= 18x10^5 cfu/ml.
If we dilute 10^4 or 10^5 that's leads to colony count of 18-19 colonies on 10^4 dilution while 2 colonies should come on plate of 10^5 dilution.
D. Or A. It’s between those two
Answer:
3.Fitness is related to whether an organism's adaptations result in more offspring
A three banded isoenzyme pattern with a 1:2:1 intensity ratio indicates a <u>dimeric</u> protein.
- Isozyme is a multimeric forms of enzyme having similar specificity of the substrate but differences in kinetics and molecular weights of the enzymes.
- The banding patterns of isozyme observed from electrophoresis is related to the genetic conditions in which different combination of polypeptide chains exhibited under the mendelian principles.
- Hence with the intensity of 1:2:1 ratio, three banded phenotypes are formed having two homomeric and one heteromeric form from the dimeric protein in both multiple alleles at single loci and multiple /single allele at multiple loci.
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<span> COMPLETE DOMINANCE:
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<span>Mendel concluded that some alleles are dominant and others are recessive. When one dominant allele is present, it's enough to make the recessive allele unexpressed (this is what happens in heterozygous individuals). In other words, it 'hides' or masks the recessive allele.
CO-DOMINANCE:
-a condition in which both alleles are dominant.
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There are alleles that have the capacity of dominating at the same time, and when an organism is heterozygotic, both alleles are expressed.
For example, a white chicken(WW) crossed with a black chicken (BB): 100% of the offspring being WB. With this genotype, they have black feathers and white feathers.
It's not a blend of colors, but a case where both are expressing.
INCOMPLETE DOMINANCE:
-a condition with none of the alleles is dominant or recessive, so the traits blend in the phenotype.
Some alleles are not completely dominant, and when that's the case the phenotype of a heterozygous organism will be a mix between the phenotypes of its homozygous parents.
For example:
plant 1: RR -red
plant 2: rr- white
By crossing this plants we will obtain 100% of the offspring with a color mix: pink.(genotype: Rr)
Red and white are not completely dominating so it results in a blend of colors.
SEX LINKED TRAITS
Sex chromosomes contain genes that determine the sex of a person. Two X chromosomes result in a female and one X plus a Y result in a male.
In those chromosomes, there are genes specific for each gender, and in those chromosomes, there are genes that code for certain traits- the sex-linked traits.
These traits will be inherited according to the sex chromosomes they receive from their parents.
