Generally speaking if time is on the x-axis and the thing that’s changing is on the y-axis, a steep curve would indicate a rapid rate of change.
Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Enzymes are proteins that catalyze biochemical reactions within cells
Tracheoesophageal fistula
Tracheoesophageal fistula (TE) is
a condition in which an abnormal channel (fistula) connects the trachea to the
oesophagus. It is a birth defect and is characterized by extensive salivation
with choking, vomiting and cyanosis during feeding. When eating, food
substances move through abnormal connection and this can cause other illnesses.
From the question given above, Morgan is likely have tracheoesophageal fistula.
Simple sugar dehydration–fragmentation products, such as derivatives of furans, pyrones, cyclopentenes, carbonyl compounds, or acids
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