There are several information's of immense importance already given in the question. Based on those information's the answer can be easily deduced.
Initial number of bacteria in the culture = 1000
Number of bacteria after t hours = 1000 (2)^t
Then
1000 (2)^t = 50000
2 ^t = 50
t = 5.6
I hope the procedure is clear enough for you to understand.
Mixture as the molecules are not bound to one another.
The short term effect of that would be stomach problems and liver damage. And thats just for fresh water. for salt water you would get shellfish poisoning, vomiting, diaherra, etc. <span />
A one-use item is disposable, because it is disposed after its use.