Answer:
In acid-fast staining, carbon fuchsin is used as a primary stain which dissolves the mycolic acid present in the cell wall of <em>Mycobacterium smegmatis </em>and penetrates through it which results in staining <em>Mycobacterium</em> red.
Staphylococcus aureus cell wall does not contain mycolic acid so carbon fuchsin does not penetrate its cell wall, therefore, it becomes colorless after destaining with acid alcohol.
After destaining step methylene blue is added to stain non-acid-fast bacteria blue. So if I mistakenly forgot to use methylene blue during the procedure <em>Mycobacterium smegmatis</em> will appear red due to carbon fuchsin present in their cell wall and S<em>taphylococcus aureus</em> will appear colorless because it is destained.
An autotroph is an organism capable photosynthesis or chemosynthesis through reducing carbon dioxide, fats, sugars and/or proteins in a repetitive cycle of energy production and use to support its life. these include all plants, algae and certain bacterium.
its counterpart is the heterotroph which is reliant on outside sources for their means of energy. aka tertiary consumer: animals, fungi and certain bacterium
1. when a earth worm eat it deposits nutrient by pooping
2. earth worm, feed grass, feed cow, human eat cow
