The best answers are deletion and insertion
All the Puritan children were taught to read, to understand the Bible, and to know the laws of the country. Reading schools were usually the only education for girls, but boys would go to grammar schools after reading schools.
Enzyme linked to the secondary antibody
an undetermined measure of antigen is joined to a surface, and after that a particular counter acting agent is connected over the surface with the goal that it can tie to the antigen. This counter acting agent is connected to a catalyst, and in the last stage a substance is included that the compound can change over to some distinguishable flag, most normally a coloration change in a concoction substrate is seen.
A disadvantage of coal to liquid production of synthetic fuels is THAT IT REQUIRES MORE COAL MINING.
The coal to liquid production of synthetic fuels involves the conversion of solid coal to liquid fuel.This can be done either directly or indirectly through chemical processes. The process has many advantages, including: improvement in national and economic security, less dependence on foreign oil, provision of some environmental benefits, such as cleaner oil. But one disadvantage of this process is that it requires extensive coal mining.
