el electrophoresis is normally set up with the negative electrode at the top of the gel and the positive electrode at the bottom
of the gel. The DNA products are loaded at the top of the gel, and then a current is applied to separate them. However, when preparing to run a gel, you accidentally switched the locations of the negative and positive electrodes such that the positive electrode is at the top and the negative electrode is at the bottom. You still loaded the DNA products at the top of the gel as normal. What result are you most likely to observe if you apply an electric current to this gel setup?
The DNA will still move towards the positive electrode
Explanation:
Gel electrophoresis is a technique which is used by the molecular biologist to separate the fragments of DNA. In the procedure, a DNA sample of a particular concentration of DNA sample composed of a particular length of DNA is loaded into the samples.
The gel is usually set up with the positive electrode at the bottom and negative at the top which by mistake when placed at the bottom and positive at the top.
The DNA will still move from the DNA wells towards the positive side as the DNA moves in the gel as the DNA posses negative charge due to phosphate groups they have and therefore move towards the positive electrode (opposite charge always).
Thus, the DNA will still move towards the positive electrode is correct.
MRNA or messenger RNA. As the central dogma of biotech goes, DNA is transcribed to mRNA which is translated to tRNA, after which the amino acids form peptide bonds and form proteins. So when you see transcription, the first thing you think of is DNA to mRNA.
