Answer:
d,d
Explanation:
all the things are all the above because they have those to help the bears survive
Are you talking about like isotonic and hypertonic or are you asking what hypotonic solution means if it was hypotonic the cell would be expanding if it was hypertonic the cell would be shrinking which means more molecules would be going inward in the cell if it was hypertonic if it was hypotonic the molecules would go more on the outside in the water would come in
Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
I believe it is the lungs.
Choice D would be your answer
