Answer:
A fusion protein can be sythesized to carry a specific tag, which is useful in identifying the proteins in various experiments. Fusion proteins with the FLAG epitope bind to specific anti-FLAG antibodies and carry this extra sequence: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. The bioteh company that sells anti-FLAG antibodies states that fusion proteins carrying the Flag epitope display the sequence on the surface of the protein. This is likely <u>A short hydrophilic 8-amino acid peptide</u> because<u> of its hydrophilic nature</u> of the eight residues are <u>Likely to be located on the surface of the fusion protein</u>, making FLAG highly <u>Specific and easily accessible for cleavage by enterokinase </u> and likely to interact<u> with other epitope and domains</u> Al with water.
Explanation:
FLAG, a short hydrophilic peptide consisting of 8 amino acids have been widely used as a fusion tag for purification and detection of a wide variety of recombinant proteins. the 3× FLAG system is an improvement upon the original system by fusing 3 tandem FLAG epitopes (22amino acids). detection of fusion protein containing 3× FLAG is enhanced up to 200 times more than any other systems.
Answer:
What happens to cells that "decide" not to divide? If the decision is to not divide, the cell goes into a quiescent, or nondividing state, called G0 (G zero).
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Answer:
Procollagen VII; COPII components.
Explanation:
The endomembrane system present in eukaryotes divides the cell organelle into the structural and functional compartment. This helps in the transport of protein, its signalling and processing of protein.
Tango1 is the packaging protein that are destined to the golgi. The interaction of procollagen VII and CopII vesicle with tango is important as these helps in the transport of tango protein to the golgi. The cop II coated vesicle is important for the transport of the protein that are destined to golgi.
Thus, the correct answer is option (B).
