Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
Answer: in order for a scientific investigation to be valid A hypothesis must be tested throughout a Scientific investigation that can gather evidence and support it.
Explanation: in any scientific investigation you have to have a testable hypothesis, without your hypothesis it’s not really a scientific investigation.
Hope this helped!
I would go with b? That just in my opinion tho
Answer:
Yes!
Explanation:
From largest to smallest: biosphere, biome, ecosystem, community, population, and organism. Hope I helped :)
It'd Be Lovely to get Brainliest .
Answer: The cell is eukaryotic because it contains mitochondria
Explanation: Because I said so but really this is the answer
