Heaps and heaps and heaps and heaps of them!
Because the chain will begin to shut down and will no longer pump [H+] (hydrogen ions) across the membrane; thus, the proton gradient can not be maintained.
:) I hope this answers your question!
Answer:
After this treatment, the investigators should expect to get a mixture of the desired enzyme, plus fragments of the peptide used to desorb the enzyme in question.
This would be the result of using a peptide as a desorption solution when the desired protein is a protease,
Assuming that the protease retains its activity in the medium in question, and that the peptide can act as a substrate (which would make sense), as the peptide solution is added, it will interact with and bind to the antibody, but some molecules will also interact with the active site of the enzyme as it desorbs and passes through, culminating on the elution of the hydrolized part of the peptide along with the enzyme.
Answer:
It is a typical Hfr mating, as trp gene is very closed to F plasmid, the Hfr bacteria donates its trp gene along with F plasmid and then process is disturbed.
Explanation:
