Answer:
The correct order is dehydration, embed in wax, cut into sections, staining
Explanation:
There are certain proceedings needed to obtain stained sections of vegetable or animal tissues for their microscope observations.
These steps are:
- Obtention of the material: The tissue is cut to an adequate size.
- Fixation: When tissues are extracted from the organism, they suffer autolysis and putrefaction, so they need to be fixated in order to keep their cells in the best state possible. Fixation involves preserving the original morphological and molecular characteristics of the tissue. Fixation avoids autolysis, putrefaction, distortion, and retraction of cells and the tissue that could affect its volume or morphology.
- Dehydration. Once fixated, the fixator must be eliminated and the tissue is dehydrated by using a gradual series of solutions with alcohol in ascendant concentrations. Dehydration must be gradual to avoid tissue deformation.
- Inclusion. To obtain thin cuts that can be observed under the optic microscope, the tissues must be included in a consistent, firm substance, that might be either hydrophilic or hydrophobic. A hydrophobic medium is paraffin wax, that provides hardness and plasticity.
- Cut. The tissue included in wax must be cut in slides or sections thin enough to allow the diffusion and penetration of light. A microtome is used to perform these cuts. When using paraffine for tissue inclusion, the cuts are about 5 to 20 micrometers of thickness.
- Stain. Once the cuts are performed, paraffin wax must be eliminated. This can be done by using an organic solvent. Then the tissue must be stained. Hematoxylin and Eosin are the most common dyes. Animal tissues in general do not have any natural color, so they need to be stained to be observed.
Answer:
Gas exchange during respiration occurs primarily through diffusion. Gas molecules move from a region of high concentration to a region of low concentration. Blood that is low in oxygen concentration and high in carbon dioxide concentration undergoes gas exchange with air in the lungs.
Explanation:
Next, Mendel took the F1 progeny and allowed them to self-fertilize. In the resulting F2 generation, 3/4 showed the dominant phenotype, and 1/4 showed the recessive phenotype. ... 1 out of 3 round pea plants from the F2 generation were true-breeding and produced only offspring with round peas.
I believe the answer is A!
They are usually held out for a period of FOUR TO SIX WEEKS after diagnosis. Splenomegaly is an infectious disease that corrupt the spleen and the enlarged spleen usually persist even after all the symptoms have disappeared. Because of this, athletes should not resume active exercise too soon. Generally, the shortest period after which it is okay to resume active exercise is four weeks after all the symptoms have gone.
