Answer:
a domain of single-celled microorganisms
Explanation:
Answer:
you want us to answer ALL those questions , cause that's alot lol but its okay if so .....
Answer:
The correct answer will be option- E. coli.
Explanation:
The organism on earth are classified in two groups- the primitive group called prokaryotes and advanced group called eukaryotes.
The prokaryotic cells are simple unicellular organisms which are not highly organized as they lack certain organelles needed to do important functions in the cell. The Eukaryotic cells are highly organized cells with organelle especially a distinct nucleus.
All organisms except bacteria and Archea are eukaryotic organism so from the given options- <em>E.coli</em> is the prokaryote as it is a bacteria and left options are eukaryotes except virus which is not considered either living nor non-living.
Thus, option-E.coli is the correct answer.
The correct answer is adding ninhydrin to developing solvent.
Ninhydrinis used to detect (color) amino-acids, since amino acids are colourless compounds. So, after development, the TLC plate is sprayed with ninhydrin reagent and dried in an oven, at 105°C for about 5 minutes. It reacts with α- amino acids that results in purple coloured spots.
The appropriate sequence of steps involved in recombinant DNA technology are as under :-
1) Selected gene is isolated.
2) Restriction enzymes cut DNA into fragments.
3) Fragments of DNA are inserted into a vector.
4) Vector and recombinant DNA multiply.
5) Recombinant DNA is inserted into host.
In the very first step, the desired gene/genetic material for cloning is isolated from the cell. To do this the cell is lysed. Cells can be lysed by sonication or using chemicals like detergents.
In the second step, isolated DNA/gene is cut with the help pf biological scalpels known as restriction enzymes. The vector (usually it is a plasmid) is also cut down using restriction enzymes.
In the third step, the fragment of the restriction digested DNA (gene) and the vector are ligated together.
In the fourth step, this vector in which desired gene has been incorporated is cloned to generate various copies.
In the last step, this recombinant DNA is inserted into a host cell where is expressed i.e. in host cell this desired gene produces its product (protein).