Answer:
The smallest size tube that will hold the entire reaction is 0.5ml
Explanation:
25ul (DNA sample) + 25ul (enzyme) + 50ul (buffer) + 400ul (water) = 500ul total
since 1000ul=1ml, then 500ul = 0.5ml
Answer:
The device that mostlikely would not be used P20
Explanation:
P20 is a pipette used to measure and sample volumes of 20ul maximum.
Answer:
The reaction mix will have 400 units of the enzyme.
The working dilution will be 0.8 Units/ul.
Explanation:
The mix has 20ul of the enzyme which is 20Units/ul. Then we have 20ulx20Units/ul= 400 Units of enzyme in the mix.
Since the final volume is 500ul, the final concentration 400Units/500ul=0.8 Units/ul.
