Answer:
DNA restriction enzymes cut the DNA molecule, while DNA ligases join the resulting DNA fragments
Explanation:
Transformation is a naturally occurring process by which bacteria incorporate exogenous genetic material from their surrounding environment. This process (transformation) is used for DNA cloning via plasmid vectors. In DNA cloning, transformation occurs after restriction enzymes cut the DNA at specific sequences named palindromic sequences (i.e, sequences that can be read the same in opposite direction). Restriction enzymes can generate sticky-ends, where enzymes make staggered cuts in the two strands (e.g., <em>BamH</em>), or blunt ends, where the resulting strands are of the same length (e.g., <em>HaeIII</em>). In general, sticky-end enzymes are more useful because they generate a 3' overhang in one molecule and a complementary 5' overhang in the other, increasing the yield and specificity of ligation. During ligation, a DNA ligase is used to join both DNA strands by forming phosphodiester bonds in the plasmid. Following transformation, bacteria can be selected on antibiotic plates.
Answer:
B or C I hope this is helpful
Answer:
The answer is A.
Explanation:
Photosynthesis stores energy in complex organic molecules, while respiration releases it.
First, deoxygenated blood flows into the right atrium of the heart from the inferior and superior vena cava.
Blood then flows through the bicuspid valve and into the right ventricle.
The blood then flows through the pulmonary valve and into the pulmonary artery. Arteries carry blood away from the heart while veins return blood to the heart. The pulmonary artery is on of the only arteries that carries deoxygenated blood.
Blood will then pass through the lungs and will be oxygenated.
After being oxygenated, the blood enters the pulmonary veins. The pulmonary veins are some of the only veins that carry oxygenated blood.
Blood enters back into the heart through the left atrium.
Then, blood flows into the left ventricle through the mitral valve.
Finally, blood is pumped through the aortic valve, into the aorta, and is distributed throughout the body.