1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
siniylev [52]
3 years ago
5

n the 1950’s, Anfinsen carried out denaturation and renaturation experiments of the protein RNAse (ribonuclease) in vitro. After

using -mercaptoethanol to reduce the disulfide links and urea to denature the protein, the protein was in an unfolded, or denatured, state. After removing the urea and the reducing agent, the protein refolded with greater than 90% activity. If the urea were removed after oxidation occurred, the protein had less than 5% activity. Which is the best explanation for why the protein would not refold correctly if the urea were removed AFTER the reducing agent was removed?
Biology
1 answer:
beks73 [17]3 years ago
5 0

Answer and Explanation:

The regular synthetic denaturant of proteins is urea. The high grouping of urea causes unfolding of protein and accordingly brings about loss of capacity of protein. The urea communicates with the protein and counteracts collapsing of protein.

During oxidation, the disufide bonds that are required for legitimate working and adjustment of protein are shaped, while in nearness of urea, the disulfide bonds are not situated effectively. The protein oxidation brings about covalent adjustment of protein that outcomes in change of physical and substance properties of protein.

The difference in physical and chemical properties of protein after oxidation and in nearness of urea can't be altered even after expulsion of urea. Along these lines, protein doesn't crease appropriately.

You might be interested in
Which organ plays the primary role in prompting individuals to seek food?
SIZIF [17.4K]
The organ which plays the primary role in prompting individuals to seek food is the brain. The brain is one of the most important organ of the nervous system. It is comparable to the CPU of a computer. It is repsonsible of processing information that it is receiving from all the senses and send the message back to the organs for a response.
8 0
3 years ago
Which statement about glaciers is true?
lions [1.4K]
C. There are two types of glaciers: Alpine and Continental

3 0
3 years ago
Read 2 more answers
Which can be left out of a food chain
jonny [76]

Answer:

option b I think I think I am not sure

6 0
2 years ago
What structure is the functional unit of contraction in a skeletal muscle fiber??
DiKsa [7]
The structure is calcium ions.
5 0
3 years ago
Which event during fertilization leads to the acrosome reaction?
Artemon [7]
<span>The release of the sperm in the vagina</span>
8 0
3 years ago
Other questions:
  • What is the difference between asexual and sexual reproduction? This is a Science question. PLZ HELP!!!!!!
    8·2 answers
  • How are particles arranged in the atom
    6·2 answers
  • Compared with small cells, large cells have more trouble doing what?
    13·1 answer
  • Scientists have altered the genetic code of some potato plants by inserting genes that make the plants produce an insecticide. W
    6·1 answer
  • What are these three types of cells and what characteristics do they have that make them that cell?
    8·1 answer
  • Michael has a substance that he puts in Container 1. The substance has a volume of 5 cubic meters. He then puts the substance in
    5·2 answers
  • The transport of specific particles through a membrane by channel proteins is known as facilitated diffusion.
    11·1 answer
  • In the formation of baby boy or baby girl, mother does not have the main role. why?​
    8·1 answer
  • An animal cell that is surrounded by fresh water
    5·1 answer
  • Food, water, living space, and diesese are examples of what
    6·2 answers
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!