In my terms, culture means how civilization(s) live, this includes language, the food they eat, their religion, and arts of architecture.
When building the Railroad, Native Americans were forced to surrender their homes and move to another place assigned by the government that would keep them out of the way of Railroad Construction. This caused thousands of Native Americans to be forced to pack their belongings and began to move with their tribes.
Some tribes attempted to fight the US for their territory however it was unsuccessful. Federal and state governments would fight to have them moved, even if it meant killing them. A lot of native americans would fight the US for their territory but majority were unsuccessful at surviving.
The answer is A.) They isolated the city-states and made them independent.
How Did Colonists React to the Proclamation of 1763? ... A desire for good farmland caused many colonists to defy the proclamation; others merely resented the royal restrictions on trade and migration. Ultimately, the Proclamation of 1763 failed to stem the tide of westward expansion.