Answer:
The correct answer will be option-C
Explanation:
Lead is a metal found in the Earth's crust which through human practices like using it in paints and other products cause lead poisoning in humans due to accumulation of lead in the body.
Lead poisoning can affect the child above 6 years and adults and prove harmful as its increased concentration can cause severe health problems common of which are the development of anaemia due to large consumption, brain damage, reduced mental growth and severe stomach ache.
Thus, Option-C is the correct answer.
Answer:
DNA restriction enzymes cut the DNA molecule, while DNA ligases join the resulting DNA fragments
Explanation:
Transformation is a naturally occurring process by which bacteria incorporate exogenous genetic material from their surrounding environment. This process (transformation) is used for DNA cloning via plasmid vectors. In DNA cloning, transformation occurs after restriction enzymes cut the DNA at specific sequences named palindromic sequences (i.e, sequences that can be read the same in opposite direction). Restriction enzymes can generate sticky-ends, where enzymes make staggered cuts in the two strands (e.g., <em>BamH</em>), or blunt ends, where the resulting strands are of the same length (e.g., <em>HaeIII</em>). In general, sticky-end enzymes are more useful because they generate a 3' overhang in one molecule and a complementary 5' overhang in the other, increasing the yield and specificity of ligation. During ligation, a DNA ligase is used to join both DNA strands by forming phosphodiester bonds in the plasmid. Following transformation, bacteria can be selected on antibiotic plates.
