Answer:
The C. elegans embryo is a powerful model system for studying the mechanics of metazoan cell division. Its primary advantage is that the architecture of the syncytial gonad makes it possible to use RNAi to generate oocytes whose cytoplasm is reproducibly (typically >95%) depleted of targeted essential gene products via a process that does not depend exclusively on intrinsic protein turnover. The depleted oocytes can then be analyzed as they attempt their first mitotic division following fertilization. Here we outline the characteristics that contribute to the usefulness of the C. elegans embryo for cell division studies. We provide a timeline for the first embryonic mitosis and highlight some of its key features. We also summarize some of the recent discoveries made using this system, particularly in the areas of nuclear envelope assembly/ dissassembly, centrosome dynamics, formation of the mitotic spindle, kinetochore assembly, chromosome segregation, and cytokinesis.
1. The C. elegans embryo as a system to study cell division
The C. elegans embryo is a powerful model system for studying the mechanics of metazoan cell division. Its primary advantage is that the syncytial gonad makes it possible to use RNA interference (RNAi) to generate oocytes whose cytoplasm is reproducibly (>95%) depleted of targeted essential gene products. Introduction of dsRNA rapidly catalyzes the destruction of the corresponding mRNA in many different systems. However, depletion of pre-existing protein is generally a slow process that depends on the half-life of the targeted protein. In contrast, in the C. elegans gonad, the protein present when the dsRNA is introduced is depleted by the continual packaging of maternal cytoplasm into oocytes (Figure 1). Since depletion relies on the rate of embryo production instead of protein half-life, the kinetics tend to be similar for different targets. By 36-48 hours after introduction of the dsRNA, newly formed oocytes are typically >95% depleted of the target protein.
Explanation:
A microscope is to see smaller things that you can’t see and it is a really good tool.
Answer:
Heredity
Explanation:
the passing on of physical or mental characteristics genetically from one generation to another
Answer:
<em>This question includes the following options:</em>
<em>A.</em><em>1 - </em><em>B.</em><em>3 - </em><em>C.</em><em>6 - </em><em>D</em><em>.8 -</em><em> E.</em><em> 12</em>
PCR which stands for <em>"Polymerase Chain Reaction"</em> is a molecular biology procedure to quickly multiply a small sample of DNA into millions or billions of DNA copies for studying purposes.
<em>"Unlinked"</em> signifies that the markers are present in 6 various chromosomes, but to amplify those markers we would need <em>(</em><em>12</em><em>)</em> unique primer sequences, due to it is required to have the reverse and forward primers for every marker; thereby the answer is <em>(</em><em>E.</em><em>)</em>
