Answer:
b. False
Explanation:
These components are not sufficient to successfully perform a PCR reaction. Deoxynucleoside triphosphates (dATP, dGTP, dCTP, dTTP) are missing so that the PCR reaction can occur perfectly.
The PCR technique allows a specific fragment of the DNA molecule to be amplified thousands of times in just a few hours. This technique revolutionized research in molecular biology because it had taken a long time for DNA amplification. From PCR it is possible to obtain enough copies of a part of DNA to detect and analyze the sequence that is the target of the study.
For a PCR reaction to be performed a solution with some components must be prepared. These components are:
- Magnesium chloride buffer (to optimize reaction and act as a cofactor for polymerase)
- Forward and reverse primers (to customize the start of the enzyme reaction)
- DNA polymerase (Enzyme required for replication of desired DNA region.)
- DNA Template (the DNA to be copied)
- PCR-grade water
- Deoxynucleoside triphosphates: dATP, dGTP, dCTP, dTTP (act as bricks in the construction of DNA molecules).
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