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2 years ago

Which of the following is a reason why sticky end producing restriction enzymes are preferred compared to blunt end ones?

Biology

1 answer:

anyanavicka [17]2 years ago

6 0

Answer:

D) Because sticky ends can be temporarily held together by hydrogen bonding between the two strands.

Explanation:

Restriction enzymes cut the DNA at specific restriction sites and by the mechanism of action they can form two types of ends:

sticky ends-single-stranded overhangs are formed
blunt ends-without overhangs.

The main advantage of sticky ends (their overhangs) is that they can complementary bind to another overhand formed by the same restriction enzyme. So, for example in cloning, if the DNA of interest and plasmid vector are cut with the same restriction enzyme, that forms sticky ends, fragment of DNA will fit into a bacterial plasmid in one direction.

On the other hand, blunt ends can be inserted into vector in both directions: head-to-tail or tail-to-head.

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2 years ago

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3 years ago

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2 years ago

Steps of the scientific method

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There are 5 steps of the scientific method

1. Make a question

2. Do some research for that question.

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3 years ago

A student thinks that because the sun is made of gas, it transfers heat to Earth by convection. Which explains why this is an in

MakcuM [25]

Answer: The space between the sun and Earth contains no matter.

Explanation:

MODES OF HEAT TRANSFER

1. Conduction: This type of heat transfer happens when there is direct contact between the two objects.

2. Convection: This type of heat transfer happens when there is a movement of fluid (liquid or gas).

3. Radiation: This type of heat transfer happens when there is direct transfer of energy through space.

Thus convection can not take place between earth and sun as there is no liquid or gas present for movement of heat. The heat is transferred from sun to earth by radiation as it occurs through space.

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3 years ago

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