On the Origin of Species was published in 1859.
C preserved bone because none of the others were enough
One of the challenges that scientists face when classifying a new fossil is that any tiny detail is all that separates one species from another. Further exploration is required to identify another set of characteristics that may help in deciding which organism belongs or create a new class.
Because only the chromosomes can be seen in a karyotype, and microdeletions or insertions are mutations at the molecular level, it is virtually impossible to detect such mutations at the chromosomal level.
3. Environment
The simplest definition of an ecosystem is that it is a community or group of living organisms that live in and interact with each other in a specific environment.