If your sleeping child eyes is moving back and forth while his eyelids are closed it is likely that he is sleep
It will be 1 and 2.
This is not biology. This is Science.
If a test tube has been used, and its contents is supposed to be disposed, carefully pour the contents to the lavatory with a confirmation of a professional (as it may damage the pipelines of the sink) and ensure that there are no splashes created that can contact the human skin. Wash the test tube thoroughly and let dry.