Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Answer: It is approximately the membrane potential at which the electrochemical gradient for Na+ is zero.
Explanation:
The charge in plasma membrane occurs with diffusion and electric fields.
For example, if there are two solutions, let's assume solution X and Solution Y are separated by a porous barrier (to diffuse in equal proportions).
The fundamental principle of diffusion states that solution from a high concentration diffuses to a lower concentration.
For instance, if the X solution contains 40 sodium ions and 40 chloride ions, the Y solution only has 30 sodium and 30 chloride ions. Due to the diffusion, these two solutions will mix and reach a steady-state.
The like charges repel, and opposite charges attract.
I have searched everywhere, but I have not found the proposals of the question, but I will explain to you what is the endoplasmic reticulum so that you can answer it.
The endoplasmic reticulum is a eukaryotic organelle located in the cytoplasm.
The endoplasmic reticulum is a network of membrane tubules (often interconnected) scattered throughout the cytoplasm of eukaryotic cells. Its membrane, which alone represents more than half of the cellular membrane system, is in contact with the nuclear envelope.
The endoplasmic reticulum can be:
Granular (or rough) (RER) that is to say associated with ribosomes.
Smooth (SER).
The granular endoplasmic reticulum is the place of synthesis (in the associated ribosomes) of the proteins secreted outside the cell and of the proteins and lipids constituting the membranes of the cellular organelles. Golgi, lysosomes, mitochondria, nucleus, ribosomes, vesicles ...). It participates in the correct folding of the proteins that have just been synthesized.
The smooth endoplasmic reticulum participates in cellular metabolism, synthesizing lipids and storing calcium.
There should be options for this question. I manged to find them elsewhere. They are:
A) the period during which tremendous growth occurs and the organs continue to develop and become functional
B) the period during which the umbilical cord develops
C) the period during which the major organs and structures of the organism first develop
D) the period during which the zygote moves down to the uterus and begins to implant in the lining
The correct answer is C. The embryonic period is the period during which the major organs and structures of the organism first develop. The embryonic period lasts from implantation of the egg in the uterus until about 8 weeks from the time of conception. It is the second major stage of prenatal development, prior to embryonic is the germinal stage and lastly is the fetal stage.
