Agar is used to assist establish an anaerobic environment that promotes nitrate reduction.
Nitrate Reduction test:
- The nitrate in the broth is converted to nitrite by organisms that can produce the nitrate reductase enzyme, which can then be further converted to nitric oxide, nitrous oxide, or nitrogen.
- Anaerobic respiration and denitrification are two processes that can convert nitrate to a variety of compounds.
- While denitrification only reduces nitrate to molecular nitrogen, anaerobic respiration employs nitrate as the bacterium's final electron acceptor, reducing it to a range of chemicals.
- The nitrate reduction test is based on the detection of nitrite and its capacity to produce a red precipitate (prontosil), which is a water-soluble azo dye, when it combines with sulfanilic acid to create a complex (nitrite-sulfanilic acid).
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The given chemical reaction given above is already balanced such that the number of atoms in the left hand side of the equation is equal to that of the right hand side. Using the dimensional analysis, proper conversion factors and the molar masses,
mass of nitrogen = (0.129 g H₂)(1 mol H₂/2 g H₂)(1 mol N₂/3 mol H₂)(28 g N₂/1 mol N₂)
mass of nitrogen = 0.602 g N₂
Therefore, 0.602 g of nitrogen will be required for he reaction.
For the calculation of number of moles of HCl
that is produced by 2.73 moles of H₂.
Considering the reaction shown below:
H₂ + Cl₂------->2HCl
This can be seen from the reaction that 1 mole of H₂ produce=2 mole of HCl
So , 2.73 mole of H_{2} will produce= 2.73\times 2 mole of HCl
That is 2.73 mole of H_{2} will produce= 5.46 mole of HCl
So 5.46 mole of HCl will be produced by 2.73 mole of H_{2}