The correct matching of enzymes and their role in the process of DNA replication is A- 3, B- 5, C-1, D-2 and E-4.
Replication is the process of synthesis of two indentical copies of DNA from a single DNA molecule. It is catalyzed by a set of enzymes with the following function-
A. RNA primase synthesizes an RNA primer (short sequence of RNA) on the lagging strand complementary to the single stranded DNA which acts as template during DNA replication.
B. Ligase is involved in joining the okazaki fragments on the lagging strand together and sealing the nicks of the DNA strand.
C. Helicase separates the two DNA strands at the replication fork.
D. Polymerase synthesizes the complementary daughter strand by adding nucleotides to DNA.
E. Topoisomerase relaxes the highly coiled DNA by creating nick ahead of the replication fork in the phosphate backbone of DNA strands.
It acts as biological catalysts, which can speed up the chemical reactions, it can fit substrates into its active site and produce a product, but the enzyme shape is not changed and it can be reused. just unless the enzyme meets high temperature or extreme pH values, which will denature them
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