Answer:
b. A second marker in the knock-out cassette, that if inserted into the genome results in cell death when plated on selective media.
Explanation:
General recombination, also known as homologous recombination, refers to the naturally occurring process of exchange of genetic material between pairs of homologous DNA sequences. This process (homologous recombination) can be exploited by genetic engineering to insert DNA segments of interest at target genes. Moreover, a cassette is a mobile DNA segment containing almost a gene and a recombination site, which is integrated into the <em>locus</em>/<em>loci</em> of interest by homologous recombination. A cassette may contain a DNA segment called 'negative marker' which prevents growth under particular conditions, while a positive marker permits growth under certain conditions. In consequence, a second marker consisting of a drug cassette may be used as a negative marker in order to evidence its insertion by inducing cell death when they are plated in selective conditions.
It would be Watson and Crick in Feb 28 1953
Answer:
Like a sperm cell, the egg contains a nucleus with half the number of chromosomes as other body cells. Unlike a sperm cell, the egg contains a lot of cytoplasm, the contents of the cell, which is why it is so big. The egg also does not have a tail.
ATP, which is created by mitochondria, (Not just mitochondria but its a good example) ADP lacks Phosphate, doesn't have enough power to transfer into adenosine triphosphate.
