Answer:
PCR is known as polymerase chain reaction used to amplify DNA sequence of our interest into multiple copies. This technique is been commonly used by researchers to study in depth about the gene of interest during their research work.
Explanation:
PCR:- It is known as polymerase chain reaction, used to amplify DNA sequence of our interest into multiple copies mainlty to millions or trillions
<u>Components</u>
Phusion HF buffer:- This buffer Create optimal reaction conditions for high fidelity amplification of DNA
dNTPs:- They are the building blocks in the synthesis of new copies of DNA.There are four dNTPs used in the amplification process that is dATP, dGTP, dCTP, dTTP. these building blocks are added in equal proportion during the PCR reaction
Phusion DNA polymerase:- This is the enzyme used in DNA amplification, it is generally a fusion of DNA binding domain to a portion of pyrococcus like proof reading polymerase. This enzyme is tolerant to various inhibitors, allowing strong amplification of DNA of interest with minimal optimization
pCD122:- Plasmid that serves as a template DNA for the amplification of desired DNA sequence of our interest.
sense primer CD474:- Sense primer is also known a reverse primer, it attaches to the stop codon of the complementary strand of DNA
antisense primer CD475:- This primer is also known as forward primer, it attaches to the start codon of the template DNA
All these components is added in such a way that the total mixture should have a reaction volume of 30.0μl
Answer:
Genes are the building blocks of heredity. They are passed from parent to child. They hold DNA, the instructions for making proteins. Proteins do most of the work in cells. They move molecules from one place to another, build structures, break down toxins, and do many other maintenance jobs.
Sometimes there is a mutation, a change in a gene or genes. The mutation changes the gene's instructions for making a protein, so the protein does not work properly or is missing entirely. This can cause a medical condition called a genetic disorder.
You can inherit a gene mutation from one or both parents. A mutation can also happen during your lifetime.
There are three types of genetic disorders:
Single-gene disorders, where a mutation affects one gene. Sickle cell anemia is an example.
Chromosomal disorders, where chromosomes (or parts of chromosomes) are missing or changed. Chromosomes are the structures that hold our genes. Down syndrome is a chromosomal disorder.
Complex disorders, where there are mutations in two or more genes. Often your lifestyle and environment also play a role. Colon cancer is an example.
Explanation: I have no clue if this is correct but I tried lol
The answer is d: all of the above! Hope this help!
