Genotype or Phenotype relationships
Answer:
Explanation:
Bacterial count in stock- 1.85x10^6 cfu/ml
Dilution methods
Take 100 uL or (0.1ml) from stock and add to 900ul (0.9ml) saline and mixed it- this makes 10^1dilution.
Now take 100ul from 10^1 dilution and add to next 900ul saline this is 10^2 dilution, similarly do upto 10^5 dilution.
Then take 100ul from 10^ 4 and 10^5 dilution seperately and plate on LB agar plate seperetely and count the colonies.
Cfu/ml formula= (No.of colonies x dilution factor)/0.1 ml
So suppose, 18 colonies formed on 10^4 dilution then total no. Of cells in stock will be 18x10^4/ 0.1= 18x10^5 cfu/ml.
If we dilute 10^4 or 10^5 that's leads to colony count of 18-19 colonies on 10^4 dilution while 2 colonies should come on plate of 10^5 dilution.
During metaphase 1 of meiosis of the cell cycle chromosomal translocation occur.
Explanation:
Chromosomal translocation is abnormal phenomenon when a fragment of chromosomes detaches and gets stick to the non-homologous chromosome in an individual. These are causative of aneuploid, cancer and infertility in organism.
In metaphase 1 of meiosis the chromosomes gets aligned on the metaphase plate. This leads to surety of equal number of chromosome in the cell.
Chromosomal translocation are seen in metaphase 1 because it is the phase of rearrangement of chromosomes where fragments easily gets attach to the other chromosome.
Answer: The subphases of interphase (in order) are G1, S and G2.
Explanation:
