Answer:
C.The added epitope disrupts the function of the tagged protein
Explanation:
When an additional sequence is tagged to a protein to use comercial antibodies, there are several reasons why this procedure wouldn't work as expected (note that we're assuming the protein is being expressed but it's not possible to detect it).
For example, the sequence of nucleotide added to codify for the tagged epitope are removed during the RNA processing. In that case, the protein would be expressed without the epitope, so it would be impossible to localize it with the antibodies.
Also, it could be that the new epitope is affecting some way the protein folding, making it not functional. This way, it would be degraded by the cell so it wouldn't be detected.
Another possibility is that the epitope doesn't affects the protein folding nor its function, but during the folding ends up in a conformation that makes it inaccesible for the antibody.
In summary, the way as the possible answers to this question are shown, the correct option seems to be C:<em> The added epitope disrupts the function of the target protein.</em>
Go somewhere where there aren't any windows
Answer:
true
Explanation:
Cerebrospinal fluids is a colorless fluid that is secreted in the choroid plexus,and circulates in the ventricles system and sub -aranchinoid space of the brain and spinal cord. its provides nutrients to the surrounding neurons, cushions the effects of trauma, pressure, acts as buffer and provides protection through immune system .
it plays a vital role in regulating blood flow, through a process called cerebral auto regulation,This is needed to maintain efficient cerebral blood flow of oxygen and other metabolites, and withdraw of metabolic wastes from the brain.
in order to analyze it to diagnose some neurological diseases its sample is usually taken out in a process called lumbar puncture.
germ cells
it occours in the germ cells
