Answer/Explanation:
Density-dependent limiting factors are non living and biotic factors that affect the size and growth of a population based on the population density, while density-independent limiting factors are those factors that do not depend on the density of a population before they can have an effect on the growth and size of the population.
Examples of density-dependent limiting factors are predation, disease, Parasitism, competition. These rely on the density of a population in order to affect the growth and size of a population.
On the other hand, examples of density-independent limiting factors are pollution, flood, temperature.
Simple version:
First, the section with the desirable gene must be identified. Assuming that has already happened, the section of DNA must be excised from the original genome using restriction enzymes, which recognize certain DNA sequences and snip DNA at those sites. DNA ligase is used to "glue" these ends back together. The DNA is inserted into a plasmid (also with restriction enzymes), which would usually contain antibiotic-resistance genes (so they survive in an environment containing the antibiotic, which would also help show if the bacteria have been successfully transformed).
Then comes the actual transformation process. The bacteria to be transformed are mixed with calcium chloride (which causes the bacteria to be more receptive to the plasmids) and then mixed with the plasmids. The bacterial cells are subjected to a heat shock (the solution is heated and rapidly cooled, e.g. by placing the mixture in a hot water bath and quickly transferred to ice) so they will take up the plasmid (since the temperature change makes the membrane more permeable). The bacteria are placed on a growth medium containing the antibiotic they're resistant to. Only those successfully transformed would survive.
It is an attributes the skin to the underlying muscle and
tissue, stocks fat for energy source, stores fat for insulation, stores fat for
cushioning muscles and bones. The deepest layer of the integument system, layer
underneath the skin, vascular, composed of adipose C.T. and areolar CT, cells
are adipocytes and fibrocytes is called Subcutaneous layer. The answer is adipocytes
and fibrocytes.
Answer:
Cork is a protective tissue that separates the living cells of the plant from the outside environment. The formation of cork in the periderm is the result of the activity of a secondary meristem, the cork cambium, or phellogen.
Explanation:
