Answer:
option A
Explanation:
In carrying out these experimental procedure, the same restriction enzyme that is used to cut out the gene of interest should also be the same used for the cutting of the plasmid to generate sticky ends. Then the desired gene is inserted into the cut plasmid then treatment with DNA ligase. Purified DNA ligase is utilized in gene cloning to join/ligate DNA molecules together forming a recombinant DNA. Thus, step 3 should be carried out after step 4.
Answer:
The sites of replication.
Explanation:
Linear DNA is and advantage for bigger organisms because there can be many places where replication can occur, otherwise, in circular DNA replication can only happen in he ORI place, that is unique. This feature allows to replicate several genes in the same amount of time being more efficient in protein synthesis.
Answer:
reflection I took the test
Protein synthesis is the process in which cells make proteins. It occurs in two stages: transcription and translation. Transcription is the transfer of genetic instructions in DNA to mRNA in the nucleus. It includes three steps: initiation, elongation, and termination.
