Answer:
Background
During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked by the primers. We included mecA-specific primers and probes in the same assay to indicate the presence of methicillin resistance in the bacterial species. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples.
Explanation:
Results
Comparison of our results with those of a conventional culture-based method revealed a sensitivity of 96% (initial sensitivity of 82%) and specificity of 98%. Furthermore, only one cross-reaction was observed upon investigating 102 culture isolates from 70 untargeted bacteria. The total assay time was only three hours, including the time required for the DNA extraction, PCR and microarray steps in sequence.
Answer: when one is lost, one is formed
Answer:
it is important that the president represents the people it is ridiculous that my friend thinks otherwise it is good that I don't think that way it is sad that many people passed from C19.
Explanation:
Each on diverged from the common ancestor and has its own accumulated mutations. If they have six differences each it would be 10,000 years per one mistake giving you a total of 60,000 years ago.
Answer:
a) Teeth arose from the rough scales of ancestral sharks.
b) Skeletal developments leading to the amphibian movement onto land probably evolved from lobe-finned fishes.
d) The anterior gill arch formed the basis for the evolution of the vertebrate jaw.
Explanation:
The animals we know today have physical characteristics that are evolutionary adaptations of characteristics of their ancestors, which have undergone a series of evolutions and modifications over the years giving rise to new species that have resulted in the species we know today. This process of evolutions and adaptations also happened with the ancestral species of fish, which allowed that today, they had characteristics such as teeth and jaws. Many of these evolutions occurred even in different species, such as the evolution that allowed the amphibians to have a skeleton, which evolved from the spine fins of fish.
