The EMB (Eosin Methylene Blue) agar is a selective and differential agar medium. It contains sucrose and lactose as fermentable substrates together with Eosin Y and methylene blue dyes which in combination gives the agar its characteristic color when prepared and serves as a pH indicator as well as an inhibitor of growth of Gram-positive bacteria. Hence, it is primarily used to isolate Gram-negative fecal coliforms while some positive fecal coliforms such as Staphylococci are also able to grow.
These coliforms are of two types:
- Lactose or sucrose fermenting coliforms form metallic sheens on the agar as a result of acid production and the response of the indicator to the increased acidity.
- Non-lactose/sucrose fermenters are only able to produce acid by protein deamination. Hence they either form colorless or pinkish colonies on the agar
On the other hand, Mannitol salt agar is a selective and differential agar medium that is primarily used to isolate Staphylococcal bacteria. The presence of sodium chloride in the medium makes it a partial or complete inhibitor of other bacteria.
Hence, an unknown bacterium that forms colorless colonies on EMB will either be a non-lactose fermenting, Gram-negative coliform that will hardly grow on Mannitol salt agar, or a Gram-positive fecal Staphylococcus.
Staphylococci bacteria are of two types:
- Coagulase-positive will form yellow colonies on the Mannitol salt agar.
- Coagulase-negative will form red colonies on the Mannitol salt agar.
More about isolating media can be found here: https://brainly.in/question/7238948
Answer and Explanation:
The steps of the sliding filament theory are:
Muscle activation: breakdown of energy (ATP) by myosin.
Before contraction begins, myosin is only associated with a molecule of energy (ATP), which myosin breaks down into its component molecules (ADP + P) causing myosin to change shape.
Muscle contraction: cross-bridge formation
The shape change allows myosin to bind an adjacent actin, creating a cross-bridge.
Recharging: power (pulling) stroke
The cross-bridge formation causes myosin to release ADP+P, change shape, and to pull (slide) actin closer to the center of the myosin molecule.
Relaxaction: cross-bridge detachment
The completion of the pulling stroke further changes the shape of myosin. This allows myosin and ATP to bind, which causes myosin to release actin, destroying the cross-bridge. The cycle is now ready to begin again.
The repeated cycling through these steps generates force (i.e., step 2: cross-bridge formation) and changes in muscle length (i.e., step 3: power stroke), which are necessary to muscle contraction.
Answer:
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Answer:
True
Explanation:
The peak of Mount Everest is made up of rock that was once submerged beneath the Tethys Sea, an open waterway that existed between the Indian subcontinent and Asia over 400 million years ago.
