Hi
Below are five major steps of DNA or gene cloning:
1: You chose the gene or specific piece of DNA you need to clone and cut the gene with restriction enzymes from the source organism.
2: You need to choose a vector for the process of cloning and you will cut the vector with same restriction enzymes through which you have cut your target DNA sequence to be inserted into the vector.
3: You place the target gene into the vector and join or seal the gene with vector by using an enzyme called DNA ligase.
4: You introduce the vector with your target gene into a suitable host organism such as yeast or bacteria through the process of Transformation. In this process host organism takes up the vector containing your target gene and starts replicating the target DNA along with their own DNA and thus creating millions of copies of target gene .
5: In the last step, the DNA or target gene is isolated from host organism and purified and is ready to be used since its quantity has been enormously increased through the process of cloning.
The cloning is also called as recombinant DNA technology and is the main process that is being used in the production of insulin for diabetes patients. You can see below image for better understanding.
Hope it help!
Answer: If one is constructing a phylogeny of reptiles using DNA sequence data of birds, mammals, amphibians or fish, the suitable outgroup to be used are mammals due to the time of divergence from other group of organisms.
Explanation: Phylogeny is used to determine evolutionary relationship between items or organisms. A phylogenetic tree is a graphical illustration of phylogenetic relationship. In phylogeny, an outgroup represent an organism that is more distantly related to other group of organisms.
In a phylogenetic tree, outgroup stands alone. It shows that the time of divergence of that particular organism is far from other group of organisms. Outgroup is used to root a tree and sometimes represent a group that is more ancestral on a tree.
It should be noted that differences in the DNA sequences of the organisms under consideration will determine which organism will serve as the outgroup.
Answer:
mitochondria
Explanation:
In aerobic glycolysis: NADH is transferred into mitochondria via the mitochondrial shuttle, where it is oxidized to NAD in the respiratory chain, ultimately producing ATP.
Speciation is b. the process by which new species are formed. They are often caused by the isolation of certain organisms in terms of environment or geographical area. This is part of the evolutionary changes that various species undergo to brought about by natural selection.
D,E,G is the correct answer
