Idk what this means sorry
Glycolysis evolved very early in ancestors that are common to all the domains of life.
This is because the site of glycolysis is cytoplasm which is present in both prokaryotes as well as eukaryotes.
As prokaryotes are the early ancestors of all domains of life therefore it is also considered that glycolysis is evolved very early.
Sickle-cell anemia is inherited..It is a condition where there are not enough healthy red blood cells to carry enough oxygen through your body...There is no cure for sickle-cell anemia over time damage is done to your vital organs...
I would say that the brain stem would automatically send a message to the muscular rib cage & your diagphragm to make them work harder.
Hope this helps :))
Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
