Eutrophication occurs due to excessive use of these compounds, which can lead to having dead zones in bodies of water.
Dead zones refers to areas with decreased levels of oxygen in the bodies of water caused by eutrophication and other factors. These affect the marine life negatively as fishes are also dependent on oxygen.
Eutrophication is defined as the presence of excess minerals in the water, which causes dense growth of plants and leads to depletion of oxygen that make the animals in the water to die.
The theory of evolution provides a number of insights:
-it tells us how the species are related
-it tells us how the species and populations react to the changes in their environment
With this knowledge, the scientists can predict how the species will react to the potential changes and support the positive changes (provide a good environment for the animals for example) <span />
Answer:
Background
During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked by the primers. We included mecA-specific primers and probes in the same assay to indicate the presence of methicillin resistance in the bacterial species. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples.
Explanation:
Results
Comparison of our results with those of a conventional culture-based method revealed a sensitivity of 96% (initial sensitivity of 82%) and specificity of 98%. Furthermore, only one cross-reaction was observed upon investigating 102 culture isolates from 70 untargeted bacteria. The total assay time was only three hours, including the time required for the DNA extraction, PCR and microarray steps in sequence.
Answer:
During metaphase, the cell's chromosomes align themselves in the middle of the cell through a type of cellular "tug of war." The chromosomes, which have been replicated and remain joined at a central point called the centromere, are called sister chromatids.
Loss of Rb, an important part of the G1-S transition checkpoint, can result in uncontrolled cell cycle progression and cancer. All of the following would mimic loss of Rb except constitutively active Ras GTPase activating protein. Correct answer: letter E.
Constitutively active Ras GTPase activating protein would not mimic loss of Rb, because it would not directly result in uncontrolled cell cycle progression.
<h3>What is Retinoblastoma (RB)?</h3>
Rb is an important tumor suppressor protein that works to inhibit cell cycle progression by preventing the activation of E2F transcription factors. Constitutively active Ras GTPase activating protein would not directly interfere with the Rb-E2F pathway, which is necessary for uncontrolled cell cycle progression and cancer.
Learn more about the cell cycle:
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