To help sort out those bacteria that have the vgp gene, scientists first attempt to grow the bacteria both in a medium with ampi
cillin and in a medium without ampicillin. demonstrate your understanding of this step by sorting the four bacterial classes based on where they will grow.
Bacteria without plasmid will only grow in the media without penicillin since it doesn't have immunity. Bacteria with recombinant plasmid with VGP gene, n<span>on-recombiant plasmid, Recombinant plasmid but no vgp gene can grow in both media(with or without penicillin) because they can withstand penicillin.</span>
They bind to an allosteric site which induces a conformational change that increases the affinity of the enzyme's active site for its substrate. This increases the reaction rate.
