Restriction enzymes can also be used to generate compatible ends on PCR products. In all cases, one or more restriction enzymes are used to digest the DNA resulting in either non-directional or directional insertion into the compatible plasmid. The most common Type II enzymes are those like HhaI (NEB #R0139), HindIII (NEB #R0104), and NotI (NEB #R0189)
Normal, clean rain has a pH value of between 5.0 and 5.5, which is slightly acidic. However, when rain combines with sulfur dioxide or nitrogen oxides—produced from power plants and automobiles—the rain becomes much more acidic. Typical acid rain has a pH value of 4.0.
<span>a large body of water surrounded by land.</span>
Phospholipids will form a bilayer in water because they contain hydrophobic (Water fearing.. in this cause water "hating") tails and hydrophilic heads (water loving). So they form a bilayer to remove the tails from water likewise, this satisfies the hydrophilic heads because they are still exposed to water.
