Answer:
C.The added epitope disrupts the function of the tagged protein
Explanation:
When an additional sequence is tagged to a protein to use comercial antibodies, there are several reasons why this procedure wouldn't work as expected (note that we're assuming the protein is being expressed but it's not possible to detect it).
For example, the sequence of nucleotide added to codify for the tagged epitope are removed during the RNA processing. In that case, the protein would be expressed without the epitope, so it would be impossible to localize it with the antibodies.
Also, it could be that the new epitope is affecting some way the protein folding, making it not functional. This way, it would be degraded by the cell so it wouldn't be detected.
Another possibility is that the epitope doesn't affects the protein folding nor its function, but during the folding ends up in a conformation that makes it inaccesible for the antibody.
In summary, the way as the possible answers to this question are shown, the correct option seems to be C:<em> The added epitope disrupts the function of the target protein.</em>
I,3,4,7 hope this will help
<span>The function of an ADP, or Adenosine diphosphate molecule, is to assist in the flow of energy throughout the cell. The basic structure of the molecule itself is made up of three main parts - a sugar backbone, which is attached one adenine molecule and two phosphate groups, which is then bonded to a 5' carbon atom of ribose. </span>
6CO2 + 6H20 + (energy) → C6H12O6 + 6O2
Carbon dioxide + water + energy from light produces glucose and oxygen.
