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nikitadnepr [17]
3 years ago
10

If you wish to use RNAi to reduce the expression of a gene in an organism. What would you inject into the organism

Biology
1 answer:
gladu [14]3 years ago
5 0

Answer:

double-stranded RNA (dsRNA) homologous to the gene of interest

Explanation:

RNA interference (RNAi) is a naturally occurring mechanism used by eukaryotic cells to suppress gene expression through regulatory non-coding RNAs (e.g., small interfering RNAs, siRNAs) that are complementary to the target gene of interest. These non-coding RNAs can bind to target messenger RNAs (mRNAs) in the cytoplasm in order to trigger mRNA degradation and/or block translation. The RNAi mechanism is widely used in molecular biology laboratories in order to inhibit the expression of target genes and thus understand gene function. The RNAi mechanism can be induced by transfecting into cells long double-stranded RNA (dsRNA) molecules that are complementary to the target mRNA sequence to be degraded. RNAi is initiated by the enzyme Dicer that cleaves dsRNAs into siRNAs (19 to 25 nucleotides), which are then incorporated into an RNA-induced silencing complex (RISC) in order to suppress gene expression at the post-transcriptional level (i.e., the mRNA is degraded after transcription).

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Lesch-Nyhan syndrome is an X-linked, recessive condition caused by mutation of the HPRT1 gene responsible for purine metabolism.
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Answer:

B. The father did not contribute a sex chromosome to his daughter due to nondisjunction of the sex chromosomes. The daughter is XO and her only X chromosome came from her mother, who was a carrier.

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Explanation:

As seen in the question above, a little girl was diagnosed with Lesch-Nyhan syndrome, which is an X-linked recessive condition caused by a mutation in the HPRT1 gene responsible for purine metabolism.

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As shown above, the father did not contribute any sex chromosomes to his daughter, which means that the daughter is XO and her only X chromosome came from her mother, who was a carrier.

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The air is full of water, as water vapor, even if you can't see it. Condensation is the process of water vapor turning back into liquid water, with the best example being those big, fluffy clouds floating over your head. And when the water droplets in clouds combine, they become heavy enough to form raindrops to rain down onto your head.

Explanation:

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Explanation: Hope it helps!

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