Merhaba is Hi... Turkum I don't know probably Turk Sizi seviyorum is I love you. I was In a Turkish school. I hope I helped you
A or c. I thought technically it was selective breeding but i haven't done genetics in a while to know what it's actually called
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Binocular helps to make distant objects appear nearer and larger
The correct answer for the above question is the ejaculatory ducts. The male urethra is divided into three regions, one of them is the prostatic urethra which is the proximal portion that passes through the prostrate gland. It receives the ejaculatory duct, which contains sperm and secretions from the seminal vesicles, and numerous ducts from the prostrate glands.
