Answer:
Deep Ocean.
Explanation:
This area recives no sunlight at all
LIPIDS are a large and varied groups of molecules that are usually not soluble in water.
<span>The main reasons why cell division is important in organisms are reproduction, repair and growth. Cell division is necessary in order for life to continue.
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The length of the cell cycle is important because it determines how quickly an organism can multiply. For single-celled organisms, this rate determines how quickly the organism can reproduce new, independent organisms. </span><span>Most of the differences in cell cycle duration between species and cells are found in the duration of specific cell cycle phases. DNA replication, for example, generally proceeds faster the simpler the organisms. One reason for this trend is simply that prokaryotic have smaller genomes and not as much DNA to be replicated.</span>
EDTA inhibits the activity of DNase during DNA isolation process.
DNase is an enzyme which degrades DNA using divalent metal ions like Mg^{2+} . These metal ions act as cofactor for the proper functioning of DNase.
EDTA chelates the divalent metal ions and thus does not allow DNase to access them.
Therefore in this way DNA is prevented from degradation during its isolation.
The ddNTP lacks the oxygen atom at 3' carbon in addition to at the 2'carbon which is lacking in the dNTP.
<h3>What is Sanger sequencing? </h3>
It is a chain termination method developed by Frederick Sanger and colleagues in 1977.
The process to determine the sequence of nucleotide bases in a part of a DNA molecule is called DNA sequencing. In the process of Sanger sequencing, the DNA molecule to be sequenced is copied multiple times by using polymerase chain reaction (PCR).
This creates DNA fragments of different lengths. Fluorescent labelled dideoxynucleotides are then used to terminate further chain formation.
This process marks the end of fragments and allows sequence determination. Therefore, a ddNTP is used in the Sanger Sequencing process. Compared to the normal DNA precursors, ddNTP lacks oxygen at the 3' carbon.
Read more about Sanger sequencing, here
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