Answer:
SEM ANSWER
Explanation:
Electron microscopy is a powerful tool in the field of microbiology. It has played a key role in the rapid diagnosis of viruses in patient samples and has contributed significantly to the clarification of virus structure and function, helping to guide the public health response to emerging viral infections. In the present study, we used scanning electron microscopy (SEM) to study the infectious cycle of SARS-CoV-2 in Vero E6 cells and we controlled some key findings by classical transmission electronic microscopy (TEM). The replication cycle of the virus was followed from 1 to 36 h post-infection. Our results revealed that SARS-CoV-2 infected the cells through membrane fusion. Particles are formed in the peri-nuclear region from a budding of the endoplasmic reticulum-Golgi apparatus complex into morphogenesis matrix vesicae. New SARS-CoV-2 particles were expelled from the cells, through cell lysis or by fusion of virus containing vacuoles with the cell plasma membrane. Overall, this cycle is highly comparable to that of SARS-CoV. By providing a detailed and complete SARS-CoV-2 infectious cycle, SEM proves to be a very rapid and efficient tool compared to classical TEM.
Answer:
C) are sequence-specific DNA endonucleases
Explanation:
Restriction enzymes represent a type enzyme capable of recognizing short nucleotide sequences to cut at specific restriction sites in the DNA, these sites are known as target DNA sequences. Some of the most commonly used restriction enzymes are <em>EcoRI</em>, <em>BamHI</em> and <em>HindIII</em>, isolated from <em>Escherichia coli</em>, <em>Bacillus amyloliquefaciens</em> and <em>Haemophilus influenza</em>, respectively. Restriction enzymes are endonucleases because these enzymes only cleave the phosphodiester bond within the DNA chain, conversely to exonucleases, which cleave nucleotides from the end of the polynucleotide DNA strand.
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Rapid environmental changes can cause:
1) Species can become vulnerable to extinction.
2) A decrease or increase of populations.
3) Diversity of species.
Diamond Abrasive and Mineral Polisher is the most useful as grains on sandpaper. According to Merriam dictionary diamond abrasive are powdered or crushed diamond used as an abrasive also called diamond powder. While mineral polisher is is a mineral that make or finish work piece through rubbing.
