Answer:
There was no sulfur compound added to the medium, that could be used as an electron donor.
Explanation:
Bacteria that use light for energy requirements mean that they get their energy from photosynthesis and transferring energy through the molecules.
Bacteria play a major part in the reductive and oxidative cycle of the sulfur which is mediated by the help of photosynthetic bacteria, so the sulfur or sulfur compounds are major requirements for their growth in the habitat or the medium.
Thus, the correct answer would be- There was no sulfur compound added to the medium, that could be used as an electron donor.
The correct answer is option C, that is, behavioral isolation.
The phenomenon of behavioral isolation takes place when two populations possess the tendency of interbreeding, but exhibit distinctions in courtship rituals or other kinds of behavior. For example, the western and eastern meadowlarks are very identical birds whose habitat overlap in the middle of the United States.
However, the members of the two species will not mate with each other, possibly as they use different songs in order to fascinate males. The eastern meadowlarks will not respond to the songs of western meadowlarks, and vice versa.
If a scientist is planning to carry out such an experiment it wouldn't be appropriate to go and test this right ahead without any serious protection. It is always advised that scientists first collect data after they've created their initial hypotheses and read the required literature.
The answer is greenhouse effect