Answer:
By way of introduction, A260/A280 ratio is use to measure Protein Contamination. This procedure was first described to measure protein purity in the presence of nucleic acids. However it is now commonly used to assess protein contamination of DNA. The method to determine the concentration of protein contamination by using A260/280 ratio method is well explained below by filling in the missing words.
Explanation:
The A260/A280 ratio method estimates protein __purity___ by measuring the absorbance maximum at 280nm caused by the amino acids__cytosine___, ____Adenine____, and ___Guanine_________ Since ___Spectrophotometer____ also absorbs in the UV range, we can correct for this contaminate by measuring the absorbance maximum at ____260nm______ and using the following equation: Concentration (µg/ml) = (A260 reading – A320 reading) × dilution factor × 50µg/ml___ When extrapolated from a standard curve, the Bradford data indicates the amount (in _ug__ ) of __unknown ___ protein found in a sample. If you know the volume of sample that was added to the assay then you can calculate the protein ____concentration ___ of the sample. The coomassie blue dye in the Bradford assay specifically binds primary ¬¬___sulfonic___ and __positive amines__ groups of the amino acid side groups of the proteins. The more the dye binds to the sample, the _anionic_ the blue color will be, and the absorbance at 595¬nm will be_shifted Amax___. A sample with an unusually __protein___ number of ___280nm of Tyrosine __ [give a specific example] amino acids will underestimate the total amount of protein present in the sample.
Answer:
There is no change, the lac l gene is constitutively expressed, regardless of the presence of lactose.
Explanation:
Lacl gene codes for the lac repressor hence is always expressed to produce lac repressors which may or may not be bounded dependent on the presence of lactose. Lacl gene expression is not regulated, thus is constitutively expressed.
Answer:
Sea shell fossils are found in a mountain-top cave in New Hampshire.
Explanation:
The answer is in asexual reproduction there is only one parent.
The larvae of a silk moth secrete the silk thread which is used to make clothes. Honey bees produce and store honey which is nutritious food. Silkworm is a creamy white colored insect which is about 2-3 cm long and shining in nature. Bee larvae have been shown to be an excellent food source for rearing insects
