1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
kakasveta [241]
3 years ago
9

To generate a knockin mouse, a scientist introduces to ES cells a construct with a mutant exon and within an adjacent intron, lo

xP sites flanking a neomycin resistance gene. What event will occur next?a. Crossing of heterozygous mice to a Cre-expressing strain.b. Homologous recombination between the mutant DNA and the corresponding gene in the mouse genome.c. Recombination between the loxP sites.d. Transplantation of cells into host blastocyst.
Biology
1 answer:
AysviL [449]3 years ago
8 0

Answer:

b. Homologous recombination between the mutant DNA and the corresponding gene in the mouse genome

Explanation:

The Cre-lox recombination system is a versatile mammalian gene-editing tool used to produce site-specific recombination events in specific cell lines (e.g., embryonic stem or 'ES' cell lines) by facilitating the integration of a circular mol­ecule in genomic DNA. This system makes use of a Cre recombinase enzyme from bacteriophage P1 that has the ability to recombine specific sequences referred to as Lox sequences. By using this system, it is possible to generate knockout mice by crossing a strain of mice expressing Cre recombinase strain with a targeted loxP-flanked (floxed) mouse strain. The generation of a floxed mouse consists of 1-generation of a targeting construct containing homologous sequences with the mouse genome and loxP sites flanking the gene sequence of interest, and 2-homologous recombination of the targeting construct with the endogenous target gene sequence in the mouse genome.

You might be interested in
Why is a hydrothermal vent precarious for life to exist?
Tcecarenko [31]

Answer:

The organisms that thrive at deep-sea vents and seeps have to survive freezing cold, perpetual darkness, high-pressure, and toxic chemicals. For this reason, they are often called "extreme"ex tr em op hi l es for the extreme nature of their living conditions. I don't know

Explanation:

5 0
3 years ago
Which of the following is an example of ecological succession?
AURORKA [14]
A. One good thing happens and other good things happen because of it.
5 0
3 years ago
How do sulfur and phosphorus move from the biotic to the abiotic pools during their cycles? A) Primary consumers eat producers.
worty [1.4K]

Answer:D) Decomposers break down the remains of producers and consumers

Explanation:A biotic pool is a tidal pool with diverse and changing habitats of living factors. We can say they are important mini ecosystems within the larger ecosystem.

The abiotic pool involves the elemental phases of nature,the non living parts of the ecosystem. Decomposition (break down) of elements/organic compounds is the characteristic bridge between both pools into what is known as nutrients.

This tell us that the ultimate dead part of producers and consumers are used up in this process.

Some of the nutrients released in the biogeochemical cyclic process includes sulfur and phosphorus and they move through the ecosystem.

Note: The biogeochemical cycle is a pathway of movement for chemical elements.

The chemical elements in nature moves through both biotic and abiotic components via abiotic and biotic interaction in the ecosystem

Few abiotic factors includes rocks, air, water, and chemicals while biotic factors includes living organisms activities.

All living and non living elements of nature play vital roles in the great biogeochemical cycle.

4 0
3 years ago
Question 4 (5 points)
Monica [59]

Answer:

The answer is Small intestine

Hope this helps

7 0
3 years ago
Which is true regarding color blindness?
777dan777 [17]
Answer will help you.

When it involves one sets of cones it is an inherited recessive trait.
4 0
3 years ago
Other questions:
  • What is the step of the scientific method that involves test of hypothesis
    13·2 answers
  • __________ is the ability to produce whatever volume the customer needs. answer correct changeover flexibility volume flexibilit
    15·2 answers
  • A rabbit is scurrying away from an approaching hawk Which part of cellular
    9·2 answers
  • Compared to most other substances, a great deal of heat is needed to raise the temperature of water by a given amount. This is b
    11·1 answer
  • How could this mRNA sequence support a triplet code? ACACACACACAC
    7·1 answer
  • The ""preservationist ethic"" is associated with ______________ .
    7·1 answer
  • This phrase, found in Darwin's Origin of Species, suggests that species adapt and change by natural selection and the changes ai
    5·2 answers
  • Adrian Raine studied the brains of violent repeat offenders and found that their brains had _____ frontal lobe tissue than the b
    5·1 answer
  • Primary productivity is the rate at which
    5·1 answer
  • What do u mean by the term inflorescence ?<br>Thank you​
    13·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!